Differential Scanning Microcalorimetry (DSC)

DSC is used to directly measure the intramolecular stability of structured macromolecules such as proteins and nucleic acids as well as the intermolecular stability of complexes such as oligomeric proteins, nucleic acid duplexes and lipid and detergent micellar systems, among other applications.


Technical data

Model – VP-DSC (MicroCal Inc)

Characteristics – Temperature range: -45 to 160 oC; Cell volume: 0.5 ml; Scanning rate variable (10 – 90 oC/h).

Applications – Thermodynamic characterization of protein and biopolymer conformational changes. Thermal stability of biomolecules (proteins, lipids, polysaccharides). Characterization of protein folding. Ligand identification. Optimization of pharmacological formations. Structure and stability of biopharmaceuticals. Determination of half-life of low molecular weight components.