Collection of samples printed onto microarrays at three Selective binding of a given target to particular
different concentrations as triplicates samples printed in the array
The microarray technology is a high-throughput tool that enables the simultaneous analyses of a large number of biomolecular interactions using very small amounts of sample. The IQFR microarray platform provides a tailor-made service that offers the possibility of building “designer” microarrays by printing in the arrays the samples of interest for a given study. A wide repertoire of samples can be printed, from purified biomolecules (proteins, lipids, or carbohydrates) to more complex samples, as cell extracts or fractions, and even extracellular vesicles and entire bacterial or yeast cells. Thus, a great diversity of functional and molecular recognition studies can be performed, covering from studies on particular receptor‒counter-receptor pairs or evaluation of compounds as inhibitors of the interaction, to analyses of large collections of clinical samples.
The approach involves three main steps, namely, preparation of samples for microarray printing, microarray printing and quality control, and microarray validation and binding assays (see Methods Enzymol 2018;598:37-70 and Methods Mol Biol 2022;2460:147-60). Manual or robotic arraying and protocols to be followed in each step are established based on the number and type of samples to be printed, and the targets to be tested.
Examples of applications are presented in the following publications:
