Characterization of lectins and lectin-glycan interactions relevant to physiological or pathological processes

Coding of bioinformation in glycans and transfer of this information via lectins is key to numerous physiological and pathological processes. Characterization of the lectins’ structure/function relationships is essential to understand their biological functions and paves the way to their pharmaceutical exploitation. A main focus is placed on galectins, a family of galactoside-binding lectins that are involved in immune and inflammatory responses, cancer and other biomedically relevant processes. Other proteins recently studied are the human macrophage Gal-specific lectin (a key receptor for the Tn carcinoma associated antigen) and different scFvs antibody fragments interacting with glycotopes involved in the rejection of xenotransplants.

Research partners: Profs. H-J Gabius (LMU Munich), J Jiménez-Barbero (CIC bioGUNE), and FJ Cañada (CIB-CSIC) and Dr. M Kilcoyne (NUI Galway).



Solís D, Bovin NV, Davis AP, Jiménez-Barbero J, Romero A, Roy R, Smetana K Jr, Gabius HJ. 2015. A guide into glycosciences: How chemistry, biochemistry and biology cooperate to crack the sugar code. 2015. Biochim Biophys Acta, 1850:186-235. PMID: 24685397

Ruiz FM, Scholz BA, Buzamet E, Kopitz J, André S, Menéndez M, Romero A, Solís D, Gabius HJ. 2014. Natural single amino acid polymorphism (F19Y) in human galectin-8: detection of structural alterations and increased growth-regulatory activity on tumor cells. FEBS J. 281(5):1446-64. PMID: 24418318

Ruiz, FM, Fernández IS, López-Merino L, Lagartera L, Kaltner H, Menéndez M, André S, Solís D, Gabius H-J, Romero A. 2013. Fine-tuning of prototype chicken galectins: Structure of CG-2 and structure-activity correlations. Acta Cryst. D: Biol. Cryst. 69: 1665-1676. PMID: 23999290

Gabius H-J, André S, Jiménez-Barbero J, Romero A and Solís D. 2011. From lectin structure to functional glycomics: principles of the sugar code. Trends Biochem Sci 36: 298-313. PMID: 21458998



Ermakova E, Millar MC, Nesmelova IV, López-Merino L, Berbís MA, Nesmelov Y, Tkachev YV, Lagartera L, Daragan VA, André S, Cañada FJ, Jiménez-Barbero J, Solís D Gabius H-J, Mayo KH. 2013. Lactose binding to human galectin-7 (p53-induced gene 1) induces long-range effects through the protein resulting in increased dimer stability and evidence for positive cooperativity. Glycobiology. 23: 508–523. PMID: 23376190

André S, Cañada FJ, Shiao TCh, Lagartera L, Diercks T, Bergeron-Brlek M, el Biari K, Papadopoulos A, Ribeiro JP, Touaibia M, Solís D, Menéndez M, Jiménez-Barbero J, Roy R, and Gabius H-J. 2012. Fluorinated carbohydrates as lectin ligands. biorelevant sensors with capacity to monitor anomer affinity in 19F-NMR-based inhibitor screening. Eur J Org Chem 23: 4354-4364. doi: 10.1002/ejoc.201200397

Solís D, Maté MJ, Lohr M, Ribeiro JP, López-Merino L, André S, Buzamet E, Cañada FJ, Kaltner H, Lensch M, Ruiz FM, Haroske G, Wollina U, Kloor M, Kopitz J, Sáiz JL, Menéndez M, Jiménez-Barbero J, Romero A and Gabius H-J. 2010. N-domain of human adhesion/growth-regulatory galectin-9: preference for distinct conformers and non-sialylated N-glycans and detection of ligand-induced structural changes in crystal and solution. Int J Biochem Cell Biol 42: 1019-1029. PMID: 20227520

López-Lucendo MF, Solís D, Sáiz JL, Kaltner H, Russwurm R, André S, Gabius H-J and Romero A. 2009. Homodimeric chicken galectin CG-1B: Crystal structure and detection of unique redox-dependent shape changes involving inter- and intrasubunit disulfide bridges by gel filtration, ultracentrifugation, site-directed mutagenesis and peptide fingerprinting. J Mol Biol 386: 366-378. PMID: 18848566

Kaltne, H, Solís D, Kopitz J, Lensch M, Lohr M, Manning JC, Mürnseer M, Schnölzer M, André S, Sáiz JL and Gabius HJ. 2008. Prototype chicken galectins revisited: characterization of a third protein with distinctive hydrodynamic behaviour and expression pattern in organs of adult animals. Biochem J. 409: 591-599. PMID: 17887955

Jiménez M. André S, Barillari C, Romero A, Rognan D, Gabius HJ. and Solís D. 2008. Domain versatility in plant AB-toxins: evidence for a local, pH-dependent rearrangement in the 2 lectin site of the mistletoe lectin by applying ligand derivatives and modelling. FEBS Lett, 582: 2309-2312. PMID: 18519041

López-Lucendo MF, Solís D, Sáiz JL, Kaltner H, Russwurm R, André S, Gabius HJ and Romero A. 2008. Homodimeric Chicken Galectin CG-1B: Crystal Structure and Detection of Unique Redox-Dependent Shape Changes Involving Intersubunit and Intrasubunit Disulfide Bridges by Gel Filtration, Ultracentrifugation, Site-Directed Mutagenesis, and Peptide Mass Fingerprinting. J Mol Biol. 386: 366-378. PMID: 18848566

Jiménez M, André S, Siebert HC, Gabius HJ, Solís D. 2006. AB-type lectin (toxin/agglutinin) from mistletoe: differences in affinity of the two galactoside-binding Trp/Tyr-sites and regulation of their functionality by monomer/dimer equilibrium. Glycobiology 16:926-37. PMID: 16774910

Jiménez M, Sáiz JL, André S, Gabius HJ, Solís D. 2005. Monomer/dimer equilibrium of the AB-type lectin from mistletoe enables combination of toxin/agglutinin activities in one protein: analysis of native and citraconylated proteins by ultracentrifugation/gel filtration and cell biological consequences of dimer destabilization. Glycobiology 15:1386-95. PMID: 16037489