Development of new microarray strategies for molecular recognition studies

 

We develop new designer’s microarrays for the study of probe features and recognition by different receptors. Using this technology, we can incorporate, and subsequently evaluate, multiple samples in a single chip, from purified molecules to whole cells. Novel microarray platforms already developed comprise a variety of natural complex systems as probes, as cell lysates, extracellular vesicles, bacteria wall fragments or entire bacteria, as well as yeast cells. Applications of these platforms include the study of specific features of the probes, as e.g. glycosylation patterns, exploration of probe recognition by antibodies, enzymes and different types of potential counter-receptors, and evaluation of compounds as inhibitors of recognized interactions. The new microarrays also serve to investigate ligand recognition by receptors present in the probes, as for example bacterial adhesins, using when necessary tailored immobilization strategies.

Research partners: Drs. JA Bengoechea (Queen’s University) and J. Garmendia (IdAB-CSIC), and Profs. E. García (CIB-CSIC) and E. Buzás (Semmelweis University).

 

Kalograiaki I, Euba B, Proverbio D, Campanero-Rhodes MA, Aastrup T, Garmendia J, Solís D. 2016. Combined Bacteria Microarray and Quartz Crystal Microbalance Approach for Exploring Glycosignatures of Nontypeable Haemophilus influenzae and Recognition by Host Lectins. Anal Chem. 88(11):5950-5957. PMID:27176788

Campanero-Rhodes MA, Llobet E, Bengoechea JA, Solís D. Bacteria microarrays as sensitive tool for exploring pathogen surface epitopes and recognition by host receptors. 2015. RSC Advances, 5: 7173-7181. doi: 10.1039/C4RA14570D