Manual glass-slide microarraying system
Model: VP 478A / 472A / 470 / 475
Features: Enables the printing of duplicate microarray slides from
96 or 384 well plates with up to 768 spots per array. Samples can
be spotted as duplicates, triplicates or more, with spot size ~500
microns in diameter (depending on slide surface), spot pitch 1,125
microns on horizontal axis and 750 microns on the vertical axis.
The pitch can be changed easily for different spot sizes by skipping
rows or columns.
Arraying system very useful for simple tests. It is composed of a microplate indexing
system (left front side of the picture), a glass-slide indexing system (right front side), a
wash and blot station (at the rear), and a 32-pin replicator (left rear). The wash and blot
station is used for thorough washing of the replicator pins between different samples.
The microplate indexing system aligns the replicator, which comprises four rows of eight
pins (32 pins in total) that have a 6-nl slot, to appropriate microplate wells.The probe
solution is transferred in the slot along with a hanging drop at the tip and on the sides of
the pin (up to 30 nl in total). The precise amount finally printed and the spot size depends
on the surface tension of both the sample solution and the slide surface.
Robotic Microarrayer
Model: Sprint
Features: Enables the printing of up to 20 microarray slides
from 96 or 384 well plates with up to 53,000 spots per slide.
The robot is equipped with a temperature and humidity
control system.
Appropriate for high-throughput assays. The robot is fitted with a 12-pin loading device that
simultaneously aspirates 12 samples from microplate wells into the print head. After loading,
sample droplets are ejected from the print head, so slides are printed on-the-fly, at very high
speed. Samples of 100 pL (or more) can be spotted, resulting in spot sizes of 100 μm in
diameter, depending on sample diffusion on the slide surface.
Microarray Scanner
Model: GenePix Autoloader 4200AL
Features: Four-color (blue, red, green and yellow) scanning
enabling a broad choice of dyes, with automatic slide handling
for up to 36 microarray slides. User selectable laser power
(5-100% laser transmission in 1% increments) PMT gain and
scan resolution (between 5 and 100 microns). Compatible with
glass slides, membrane-coated glass slides and embedded
arrays and a wide variety of samples (carbohydrate, protein,
gene, tissue and cell arrays).
The scanner is equipped with four color (blue, red, green, and yellow) lasers, enabling the
scanning of a broad choice of dyes. The scanner is controlled with the GenePix Pro
software, which combines all the scanner control, imaging, and analysis tools required for
scanning, producing microarray pictures in exportable formats and quantitating the
fluorescent signals.
Other equipment
Slide holders for dividing the slides into separate incubation chambers. Different modules are available with 1 to 16 chambers, thus enabling performing up to 16 independent binding assays simultaneously. The modules can be used individually or can be fitted into a tray with capacity for 1–4 slides, thereby building a modular plate. The holder is fixed over the microarray slide with lateral clips. After the assay, the module is disassembled and the slide is scanned.
A)Slide holder FAST® frame for up to four slides, single-pad B)ProPlate® slide module for an individual
nitrocellulose-coated glass slide, and suitable incubation chamber. 16 pad slide, coupled (left) and uncoupled (right).