Spectrofluorimetry

Technical data

Model – Fluormax-4 (Horiba Jobin Yvon)

Characteristics – Two detectors (one is a calibrate photodiode reference detector to correct for intensity and temporal fluctuations in the source during excitation scans, the other is used in photon counting with signal linearity up to 2 x 106 CPS). Wavelength range: 220-600 nm for excitation and 290-850 nm for emission. Temperature range: 10 to 100 oC (peltier control system). Solid sample holder designed for samples such as thin films, powders, pellets, microscope slides and fibers.

Applications – Fluorescence spectroscopy (excitation, emission, synchronous scans). Temperature-wavelength scan (monitoring a range of wavelengths as a function of temperature). Kinetic scans.

Main application of these methods include: studying protein tertiary structure, protein unfolding as a function of temperature or added chemical denaturants, characterization of the effects of mutations on protein or nucleic acid structure and stability, studying macromolecule–ligand, macromolecule–macromolecule interactions, and kinetics processes. Micro-viscosity of membranes. Studying solid simples, films and fibers.