PRICE €

IQFR

CSIC

AGE/OPIs/                   Universities

Other users

Microarray printing of bacteria or exosomes (up to 48 samples) and binding assays (up to 32 samples)	250,19	293,48	1349,39	1477,90
Microarray printing (except bacteria) [2 slides of 16 pads, 24 samples printed per pad] and binding assay (up to 32 samples)	213,75	250,75	809,39	886,48
Manual microarray printing [2 single-pads slides, 24 samples printed] and binding assays (2-6 samples)	145,99	171,24	682,07	743,03
Additional microarray printing (either bacteria or not) and binding assays (up to 32 samples)	146,18	171,47	741,45	812,07
Additional manual printing [2 single-pad slides, 24 samples printed] and binding assays (2-6 samples)	137,60	161,41	533,83	584,01

 

 

*IVA not incluided

 

 

  Manual glass-slide microarraying system

 

 

Arraying system very useful for simple tests. It is composed of a microplate indexing

system (left front side of the picture), a glass-slide indexing system (right front side), a

wash and blot station (at the rear), and a 32-pin replicator (left rear). The wash and blot

station is used for thorough washing of the replicator pins between different samples.

The microplate indexing system aligns the replicator, which comprises four rows of eight

pins (32 pins in total) that have a 6-nl slot, to appropriate microplate wells.The probe

solution is transferred in the slot along with a hanging drop at the tip and on the sides of

the pin (up to 30 nl in total). The precise amount finally printed and the spot size depends

on the surface tension of both the sample solution and the slide surface.

 

 

 

 

 

 

 

 

 

 

 Robotic Microarrayer

 

 

Appropriate for high-throughput assays. The robot is fitted with a 12-pin loading device that

simultaneously aspirates 12 samples from microplate wells into the print head. After loading,

sample droplets are ejected from the print head, so slides are printed on-the-fly, at very high

speed. Samples of 100 pL (or more) can be spotted, resulting in spot sizes of 100 μm in

diameter, depending on sample diffusion on the slide surface.

 

 

 

 

 

 

 

 

 

 

 

 

 

 Microarray Scanner

 

 

The scanner is equipped with four color (blue, red, green, and yellow) lasers, enabling the

scanning of a broad choice of dyes. The scanner is controlled with the GenePix Pro

software, which combines all the scanner control, imaging, and analysis tools required for

scanning, producing microarray pictures in exportable formats and quantitating the

fluorescent signals.

 

 

 

 

 

 

 

 

 

 

 

 

Other equipment

 

Slide holders for dividing the slides into separate incubation chambers. Different modules are available with 1 to 16 chambers, thus enabling performing up to 16 independent binding assays simultaneously. The modules can be used individually or can be fitted into a tray with capacity for 1–4 slides, thereby building a modular plate. The holder is fixed over the microarray slide with lateral clips. After the assay, the module is disassembled and the slide is scanned.

     

A)Slide holder FAST® frame for up to four slides, single-pad                   B)ProPlate® slide module for an individual

   nitrocellulose-coated glass slide, and suitable incubation chamber.      16 pad slide, coupled (left) and uncoupled (right).
                                                                                                                         

                                                                                        

 

 

 

The microarray facility provides a tailor-made service that offers the possibility of building “designer” microarrays and running a diversity of binding analyses. Applications may include, among others, the characterization of particular features of the printed samples (referred to as probes), screening of the binding properties towards particular probes of samples of interest (referred to as targets), or the evaluation of compounds as inhibitors of probe‒target interactions.

To design a customized service for meeting the users’ needs, a description of the pursued objectives and probes/targets to be tested should first be sent to This email address is being protected from spambots. You need JavaScript enabled to view it.. The user will next be contacted by the service for discussing specific details, as e.g. number of slides and amounts of probes/targets expected to be required for the intended analyses, possible systems for validation of microarray printing and detection of binding, and scheduling of the assays. As an orientation, about 1 mg of probe or 1 ml of bacterial suspension at OD₆₀₀ = 1 is required for screening recognition by multiple targets, while 50-100 µg of a given target is required for testing its binding preferences towards different printed probes.

In standard procedures, the biotin-streptavidin system is used for detection of binding. To this aim, the microarray service provides Alexa Fluor 647-labeled streptavidin and different primary and secondary antibodies. The service also offers libraries of glycoproteins and (lipo)polysaccharides that can be used as well-defined probes, a panel of over 40 plant lectins of known carbohydrate-binding specificities for the analysis of glycosylation patterns of test probes, and a broad collection of human lectins of the innate immune system for evaluating the potential of test probes to serve as ligands for these lectins.

Once all details are fixed and agreed, a formal Request Form should be filled and sent to the service (This email address is being protected from spambots. You need JavaScript enabled to view it.). Additional information on the samples may be requested at this stage.

A report detailing the assays carried out and the results obtained, in the form of images and tables with fluorescence binding intensities, will be sent to the user by email. Data will remain confidential until publication or protection. Thorough analysis and discussion of the results on a collaborative basis can be agreed.

 

 

 

 

 

 

 

MICROARRAY SERVICE

 

 

 

 

The microarray technology is a high-throughput tool that enables the simultaneous analyses of a large number of biomolecular interactions using very small amounts of sample. The IQFR microarray platform provides a tailor-made service that offers the possibility of building “designer” microarrays by printing in the arrays the samples of interest for a given study. A wide repertoire of samples can be printed, from purified biomolecules (proteins, lipids, or carbohydrates) to more complex samples, as cell extracts or fractions, and even extracellular vesicles and entire bacterial or yeast cells. Thus, a great diversity of functional and molecular recognition studies can be performed, covering from studies on particular receptor‒counter-receptor pairs or evaluation of compounds as inhibitors of the interaction, to analyses of large collections of clinical samples.

The approach involves three main steps, namely, preparation of samples for microarray printing, microarray printing and quality control, and microarray validation and binding assays (see Methods Enzymol 2018;598:37-70 and Methods Mol Biol 2022;2460:147-60). Manual or robotic arraying and protocols to be followed in each step are established based on the number and type of samples to be printed, and the targets to be tested.

 

 

   

  

  

 

 

Examples of applications are presented in the following publications:

Vanacore A, Vitiello G, Wanke A, Cavasso D, Clifton LA, Mahdi L, Campanero-Rhodes MA, Solís D, Wuhrer M, Nicolardi S, Molinaro A, Marchetti R, Zuccaro A, Paduano L, Silipo A (2022) Lipopolysaccharide O-antigen molecular and supramolecular modifications of plant root microbiota are pivotal for host recognition. Carbohydr Polym 277:118839 (doi: 10.1016/j.carbpol.2021.118839).

Campanero-Rhodes MA, Kalograiaki I, Euba B, Llobet E, Ardá A, Jiménez-Barbero J, Garmendia J, Solís D (2021) Exploration of galectin ligands displayed on gram-negative respiratory bacterial pathogens with different cell surface architectures. Biomolecules 11:595 (doi: 10.3390/biom11040595).

Campanero-Rhodes MA, Lacoma A, Prat C, García E, Solís D (2020) Development and evaluation of a microarray platform for detection of serum antibodies against Streptococcus pneumoniae capsular polysaccharides. Anal Chem 92:7437-43.

Kalograiaki I, Euba B, Fernández-Alonso MDC, Proverbio D, St Geme JW 3rd, Aastrup T, Garmendia J, Cañada FJ, Solís D (2018) Differential recognition of Haemophilus influenzae whole bacterial cells and isolated lipooligosaccharides by galactose-specific lectins. Sci Rep 8:16292 (doi: 10.1038/s41598-018-34383-x).

Kalograiaki I, Abellán-Flos M, Fernández LA, Menéndez M, Vincent SP, Solís D (2018) Direct evaluation of live uropathogenic Escherichia coli adhesion and efficiency of anti-adhesive compounds using a simple microarray approach. Anal Chem 90:12314-21.

Bustamante N, Iglesias-Bexiga M, Bernardo-García N, Silva-Martín N, García G, Campanero-Rhodes MA, García E, Usón I, Buey RM, García P, Hermoso JA, Bruix M, Menéndez M (2017) Deciphering how Cpl-7 cell wall-binding repeats recognize the bacterial peptidoglycan. Sci Rep 7:16494 (doi: 10.1038/s41598-017-16392-4).

Kalograiaki I, Euba B, Proverbio D, Campanero-Rhodes MA, Aastrup T, Garmendia J, Solís D (2016) Combined bacteria microarray and quartz crystal microbalance approach for exploring glycosignatures of nontypeable Haemophilus influenzae and recognition by host lectins. Anal Chem 88:5950-57.

Campanero-Rhodes MA, Llobet E, Bengoechea JA, Solís D (2015) Bacteria microarrays as sensitive tools for exploring pathogen surface epitopes and recognition by host receptors. RSC Adv 5:7173-81.